EIF4A1 Antikörper (N-Term)

Produktnummer ABIN7603144

Der Maus Monoklonal Anti-EIF4A1 Antikörper wurde für WB, IF, ICC, IHC und FACS validiert. Er ist geeignet, EIF4A1 in Proben von Human, Ratte und Maus zu detektieren.

Kurzübersicht für EIF4A1 Antikörper (N-Term) (ABIN7603144)

Target: EIF4A1 (Eukaryotic Translation Initiation Factor 4A1 (EIF4A1))

Reaktivität: Human, Ratte, Maus

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser EIF4A1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Flow Cytometry (FACS)

Klon: 3F11

Produktdetails anti-EIF4A1 Antikörper

Bindungsspezifität: N-Term

Verwendungszweck: Anti-EIF4A1 Antibody Picoband® (monoclonal, 3F11)

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-EIF4A1 Antibody Picoband® (monoclonal, 3F11) (ABIN7603144). Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human EIF4A1, identical to the related mouse and rat sequences.

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human, Mouse, Rat
1. Garcia-Garcia, C., Frieda, K. L., Feoktistova, K., Fraser, C. S., Block, S. M. Factor-dependent processivity in human eIF4A DEAD-box helicase. Science 348: 1486-1488, 2015. 2. Jones, E., Quinn, C. M., See, C. G., Montgomery, D. S., Ford, M. J., Kolble, K., Gordon, S., Greaves, D. R. The linked human elongation initiation factor 4A1 (EIF4A1) and CD68 genes map to chromosome 17p13. Genomics 53: 248-250, 1998. 3. Kim, N.-S., Kato, T., Abe, N., Kato, S. Nucleotide sequence of human cDNA encoding eukaryotic initiation factor 4A1. Nucleic Acids Res. 21: 2012 only, 1993.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Details zu EIF4A1

Target: EIF4A1 (Eukaryotic Translation Initiation Factor 4A1 (EIF4A1))

Andere Bezeichnung: EIF4A1

Hintergrund:

Synonyms: SUMO-activating enzyme subunit 2, Anthracycline-associated resistance ARX, Ubiquitin-like 1-activating enzyme E1B, Ubiquitin-like modifier-activating enzyme 2, UBA2, SAE2, UBLE1B, HRIHFB2115

Background: Eukaryotic initiation factor 4A-I is a protein that in humans is encoded by the EIF4A1 gene. It is mapped to 17p13.1. EIF4A1 has been shown to interact with EIF4E and eukaryotic translation initiation factor 4 gamma.

Molekulargewicht: 47 kDa

Gen-ID: 1973

UniProt: P60842